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TinLizzy
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Date Posted:06/23/2010 1:35 PMCopy HTML

http://themaddiecasefiles.com/topic3839-920.html


For those of you who tweet and twitter, please feel free to use this, whether redacted, plagiarised, cropped, chopped, sent to PRO sites or otherwise. Your contributions are welcomed in ripping it to pieces or adding things to it.

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Every so often we come across a word or a phrase that stands out - and sticks in our minds.

''A match between a crime scene sample and an individual would be a very rare event if the individual was not the true source of the crime scene sample.''

This investigation should have been trying to determine what happened to a missing child that includes, not excludes, all trace evidence found - and that includes biological material that indicates where the child, or the body of the child may have been after she went missing.

Any police assessment done regarding Madeleine would have classified her as 'High Risk' - The risk posed is immediate, or there are substantial grounds for believing that the subject is in danger through their own vulnerability.

Here is part of the guidance on the management, recording and investigation of missing persons from the ACPO - Association of Chief Police Officers – National Centre for Policing Excellence. Very comprehensive guidance .

The general principals for search & evidence gathering and early intervention for missing persons who are classified as at risk are as follows;

The enquiry has to consider capturing physical evidence which might assist in the identification and recovery of bodies or future enquiries into homicide . This means obtaining identifying characteristics for reference from objects left behind. This process is not simply collection .

Investigative effort must be made and recorded to establish as far as possible, the link between the recovery and the missing person . The most likely sources are;

DNA profiling – For this to happen the missing person must be considered the subject.
Blood grouping .
Fingerprints & palm prints
Dental Chart Records .

At the scene of a crime, samples are collected from the surrounding area, and this can be for the purposes of eliminating individuals from police enquiries as well as to help narrow down the list of suspects or victims.

Trace Evidence Analysis is the discipline of forensic science that deals with minute transfers of materials ( DNA ) that cannot be seen with the unaided eye.

The results of a DNA analysis can provide an important link between victim, suspect, and/or crime scene, and can also conclusively exclude or include an individual as being the source.

The key to DNA evidence lies in comparing the DNA retrieved from the scene of a crime. To do this, investigators have to do three things:

1.Collect DNA at the crime scene.
2. Analyze the DNA to create a DNA profile.
3. Compare profiles to each other.

The effective use of DNA as evidence may also require the collection and analysis of elimination samples to determine the exact source of the DNA.
Elimination samples may be taken from anyone who had access to the crime scene and may have left biological material.

These points are extremely important in this case and something else to keep in mind .

Authorities can extract DNA from almost any tissue, including hair, fingernails, bones, skin, teeth and bodily fluids.

DNA samples can be generated by tiny amounts of tissue.
DNA is the genetic material found within the cell nuclei of all living things.
DNA is a long molecule and DNA fingerprinting relies on the fact that specific bits of this molecule are replicated in little clusters along its length.

It is this sequence and number of repeats which is a useful way of distinguishing one individual from another.

A DNA 'fingerprint' relies on the fact that hardly anyone will share the same pattern of repeats along the DNA molecule. If this happens, then the chances that they are from two different individuals is very slim.

There are only ever 3 types of results obtained from DNA analysis - Conclusive/Inclusive - Exclusive - Inconclusive.

Inclusion: When the DNA profile of a victim or suspect is consistent with
the DNA profile from the crime scene evidence.

Exclusion: When the DNA profile from a victim or suspect is inconsistent
with the DNA profile generated from the crime scene evidence.

Inconclusive: Inconclusive results indicate that DNA testing could neither
include nor exclude an individual as the source of biological evidence.

In mammals, the strands of DNA are grouped into structures called chromosomes.
With the exception of identical twins, certain sequences of DNA of each individual are unique.
A DNA fingerprint is constructed by first extracting a DNA sample from body tissue or fluid.

The sample is then segmented using enzymes, and the segments are arranged by size using a process called electrophoresis.

The segments are marked with probes and exposed on X-ray film, where they form a characteristic pattern of black bars – called a DNA fingerprint.

To identify individuals, forensic scientists scan 10 DNA regions, or loci that vary from person to person and use the data to create a DNA profile of that individual (the DNA fingerprint). There is an extremely small chance that another person has the same DNA profile for a particular set of 10 regions.

A nuclear DNA match of loci permits little doubt that a questioned sample has come from a known individual, except in the case of identical twins.

On average, two people would probably have six or seven DNA markers in common out of 20, simply by chance. But with over 12/13 bands in common, you very, very rarely see unrelated people with that degree of similarity.

If the DNA fingerprints produced from two different samples match, the probably of two samples being from the same person is extremely high .

Generally, courts have accepted the reliability of DNA testing and admitted DNA results into evidence.

An example of conclusions provided to the Courts when a DNA match is observed would be as follows:

Approximately 1 person in every 5 trillion chosen at random from the population would be expected to possess the same DNA genotype as that found in a questioned sample . Since 5 trillion is much less than the population of the World (and so one couldn’t have 5 trillion people to compare) an alternative conclusion (based upon the same data) may be adduced. The DNA results are 5 trillion times more likely if the questioned sample originated from the suspect, than if it had originated from a randomly chosen unrelated individual from the population.

Tiny amounts of Madeleine's DNA which can last for many years without substantially degrading are probably present on just about everything the family has, including toys, Madeleine’s clothes, their clothes, their furniture and in their car etc. Believe it or not we lose/shed approx. 4000 to 5000 skin cells every minute, and each one is unique to our very own personal identity .

As you are probably all aware, a genetic profile of Madeleine was done initially by using samples from both her parents. This type of profile is called a familial profile * and would show, when compared to one of their children’s DNA profiles, enough components to prove whether or not a child was theirs ........... But that’s all it would do. It would not prove which child it was, as everyone barring identical twins, all have DNA components that are unique to us, and make us all different .

It wasn't possible to use the same method to create an actual genetic profile for Madeleine, so instead of attempting to create one from genetic evidence found on items of clothing she wore; toys she played with; or anything else personal to her that she used in PDL, the authorities went back to the UK to Rothley to try and find a sample from her home, and found the stain on a pillow case. ( Believed to be saliva ) Ref. (SJM/1) in the FSS report .

So we know they created two genetic profiles. One created from the DNA taken from her parents (a Familial profile sample) and one created from the stain found on the pillowcase in Rothley, which matched a blood sample which we assume was from a 'heel stick sample'.

Just to clarify, these two genetic profiles would not have been identical because only one would show the unique DNA components which only MM had in her genetic make up.

The need for the blood or heel stick sample (if that’s what it was) is understandable for definite comparison purposes, but, call me an old cynic, I am naturally suspicious that this was not, or could not be compared to DNA found both in the apartment and on anything belonging to Madeleine from PDL.

They were successful in locating a stain on a pillow case which is believed to be saliva, ( ref SJM/1 ) and this was compared to reference samples of Madeleine’s immediate family, and proved to be different .

On 12 October 2007, the Forensic Science Service received a blood spot in a cardboard frame (object JRB/1) from Leicestershire Constabulary. That object was inside a sealed package.
The DNA profile was the same as that obtained from possible spots of saliva existing on the pillowcase, and thus was born a true genetic profile of MM .

The FSS confirmed this by stating, 'The results of the DNA profile obtaïned from the pïllowcase is approximately 29 million times more likely if the profïle originates form a natural child of theirs, rather than someone unrelated to them.' ........... Can't really argue with that.

I’ve no doubt the heel stick sample was necessary in order to provide them with a definite genetic profile to compare to the Rothley sample, and that a true profile was assembled as a result.

I’ve heard various suggestions as to why this might have occurred. Everything from the police & forensics not initially looking for a specific sample of her DNA in PDL, to attempts to wipe out any evidence of her existence.

Whatever the case, one thing is certain, her DNA should have been present on many things and in many places. They even had a sticker book belonging to her, perfect I would think, for collecting a DNA sample from.

So if the heel prick sample was compared to a sample found in Rothley, (the pillowcase sample) and proved conclusively to be from Madeleine - but nothing was found in PDL in real terms. This means we still do not know for certain if the child who was in PDL has the same DNA profile or was in fact even the same child.

There is no genetic evidence to prove the profile compiled from both the heel prick and pillowcase is compatible to any DNA from the child in PDL. We simply don't know if any DNA from clothing, toys, toothbrush, or anything else she used in PDL matched that profile.

I would have thought most intelligent people whose child goes missing, would try to preserve some sort of evidence (worn clothes) belonging to the child. More for emotional reasons rather than for forensic purposes.

Worn clothing is the most obvious choice when no other biological sample is available. As I’ve mentioned, humans shed aprox. 4000 – 5000 skin cells a minute. Each one unique to that person - not to mention other biological stains/traces that would have been present on items of clothing. In an early report publicized in the press, it was said that a white soiled sock supposedly belonging to Madeleine was sent to the FSS in order to extract a sample of her DNA - though we have never seen any report about this in the files.

Realistically, there should have been an ample supply of worn clothing - and we’ve seen two photographs supposedly taken on May 3rd of her dressed in two different outfits – In the tennis ball pic, and the famous last photo showing her by the pool. Not to mention other items where her DNA should have been present, and should have been as easily collectable as any sample from Rothley.

I have to admit at this stage, like so many people, I’m baffled by the results and conclusions of the FSS report - in so much as results should have provided an important link between victim, suspect, and/or crime scene. They can also conclusively exclude an individual as being the source of the evidence – This is another point I believe is particularly relevant to this case, because that is what appears to have happened.

I’d like to just refer to a Letter dated 11 September regarding FSS report received by the PJ on 4 September from Leicester Police, citing 15/19 matches of Madeleine DNA profile [/b]

This serves to add [to the case file] a laboratory examination report prepared
in England, written in English and translated into Portuguese, delivered to this
police force on 4 September 2007 by English police officer Stuart Prior.

This laboratory report tells about the examinations made of two trace evidence
recoveries, one from behind the living room sofa in apartment 5A, and the other in the boot area of the vehicle used by the McCann family, hired [by them] from the end of May this year.

In some of these recoveries (samples) DNA was found whose components are also
found in the profile of Madeleine McCann.

With respect to the trace evidence recovered behind the sofa, all the confirmed
DNA components coincide with corresponding components in the DNA profile of
Madeleine McCann.

In the sample collected in the boot area of the vehicle, 15 of the identified
DNA components coincide with the corresponding components in the DNA profile of
Madeleine McCann, this of [having] 19 components.

Portimao, 11 September 2007

* ‘Familial‘ searching.
Matches parents to children. Standard DNA profiles examine 10 markers in the DNA. Each marker has two sequences - one inherited from the mother, and one from the father.
Familial searching is based on the way in which DNA is inherited within a
particular family group. DNA profiles of individuals who are related to each
other being more likely to contain similarities in their DNA profiles than two unrelated individuals.

Clearly something changed, as the interpretation of the results by John Lowe attempted to exclude MM as being someone who contributed to the samples found. – I say attempted, because he failed and ended up contradicting himself.

“An incomplete DNA result was obtained through LCN from cellular material present in the swab (286A/2007 CRL 3A). The low-level DNA result showed meagre information indicating more than one person. Departing from the principle that all confirmed DNA components within the scope of this result originated from a single source, then these pointed to corresponding components in the profile of Madeleine McCann. However, if the DNA within the scope of this result originated from more than one person, then the result could be explained as being DNA originating from [a mixture of DNA from both] Kate Healy and Gerald McCann, for example. DNA profiles established through LCN are extremely sensitive; it is not possible to attribute this DNA profile to a particular body fluid. Nor to determine how or when that DNA was transferred to that area."

"DNA analysis uses a technique in which specific regions [areas] are seen and copied (or amplified) many times. A DNA profile obtained from biological material, such as blood, semen, saliva or hair may be compared with a DNA profile obtained from a reference sample of any person. In the case that the DNA profile of the particular person is different from the DNA profile of the biological material, then that person is not the source of that material. If the profiles are equal [match], then that person, together with other persons having the same DNA profile, may be considered as a potential source of the material."

This investigation should have been trying to determine what happened to a missing child, that includes - not excludes all trace evidence found; that includes biological material that indicates where the child or the body of the child may have been after she went missing. The cellular material found, contained enough DNA components to fit the profile of the missing child, and the report clearly states she should have been considered as a potential source of the material/ sample , so why was she not?

Coincidently, it appears the GBFS – seem to have done the same thing with hair samples. 12 hairs were recovered from tops belonging to Madeleine; nothing from the hairbrush; nothing from the pillowcase or anywhere else we know of. These were used as substitute reference samples of her hair because others found were not considered to be authentic samples …........But they were considered not representative of a sample of her hair because they didn't match photographs of her hair .......... or were too short in length to do mtDNA tests .

‘A total number of twelve [12] hairs or hair fragments were recovered from the tops SJM/2, SJM/4 and SJM/5. All of these appeared to be hair and not down, being mainly blonde in colour. One of the hairs was brown and distinctly darker than the other hairs, suggesting at the least, that this was a hair from someone else.‘

‘Conclusion
In the objects recovered from the Scenic, there were around 15 blonde/fair hairs similar to the reference hairs from SJM2, 4 and 5. However, as it was not possible to do solid [definitive] or significant [forensically meaningful] tests, it is not possible for me to determine if, or not, these could have been from Madeleine McCann.’

‘Approximately 15 hairs, down or fragments were blonde and fair, presenting a similarity with the reference material. All were of insufficient length to make a solid [definitive] comparison. Furthermore, they are too short to do mitochondrial DNA tests ‘

'The remaining eleven hairs/fragments varied in length from 4 millimetres to 45 millimetres [~1/8" to ~1,3/4"]. I could not conclude that all hairs were from the same person. If they had been from Madeleine McCann, then they are not representative/typical/characteristic of a sample of her hair, given the length of that seen in photographs of her.’

There were more than two hundred hairs, down or fragments of hair collected . The majority appeared to be different from the blonde reference hairs recovered from SJM2, 4 and 5. Furthermore, no blonde hairs consistent with that seen in photographs of Madeleine McCann were found.

No hair was recovered from the pillow-case SJM/1, nor the hairbrush SJM/36.

So no hairs found that belonged to Madeleine, not even the 12 hairs found on the 3 tops she wore, and apparently no other DNA samples that we know of obtained from PDL either.

How strange is that? - Not only has Madeleine's’s body vanished - but all genetic traces of her seems to have just vanished from PDL ?

Or have they?

At this juncture, let's not forget man's best friends Eddie & Keela, because I don't think they were wrong, but what did they find if there was no evidence of Madeleine?

Whose body and DNA was behind the sofa in the apartment, and whose body & DNA was in the hire vehicle ?

What a conundrum !!

Forensic evidence dogs don’t look for live scent, so whoever commissioned these dogs to search, did so to try and find evidence of human remains .

The EVRD is trained to located the scent of a dead body. The CSI dog is trained to find human blood, and blood is in fact human remains .

So were the dogs correct and did the FSS and the GBSF deliberately mislead the general public?

Were there actually samples of hair belonging to Madeleine, and if so why hide that fact ?

Was there DNA evidence in the apartment and on her clothes etc., and if so why disguise the fact.

Was the evidence the forensic /biological evidence found as a result of the specialist dogs really Madeleine's, and if so why claim it was inconclusive?

What we're left with are 5 considerations;

1.) Either the Forensic results and conclusions were tampered with to get the Mc's off the hook

2.) The FSS & GBFS are useless and botched the whole thing up

3.) The results are factual and there was no DNA or hair samples belonging to MM in PDL

4.) There is something about Madeleine's's DNA that caused the results to vary.

5.) the DNA and hair samples belonged to someone other than Madeleine who was obviously related to the Mc's

If the results were tampered with, what hook did they need to get the Mc's off?

If the results are correct, and there was no DNA or hair evidence belonging to Madeleine in PDL, then we have to consider the last two possibilities.

It's possible a genetic defect could have caused a variation in the DNA sample, blood chimeraism might account for this or some other genetic problem, but would it account for the mystery of the hair samples?

So that comes down to no.5 which could tie in to no 1 ............

''A match between a crime scene sample and an individual would be a very rare event if the individual was not the true source of the crime scene sample.''

A rare event. What constitutes a rare event? Could it be something to do with something or someone special ?

Could there be something that made someone very special ?

Someone and something so special it would be like opening Pandora's Box if the truth ever came out ?

One thing is certain, there was according to the GBFS, no hairs found in PDL belonging to Madeleine.

They do not appear to have recovered any genetic/DNA evidence in PDL belonging to Madeleine.

The DNA evidence found as result of the EVRD and CSI dogs proved to be inconclusive according to the FSS .

This, I believe, narrows things down to 2 other considerations;

1. There is no evidence other than the word of the Mc's family and their friends and tampered photographs to prove it was definitely Madeleine in PDL - Realistically when we piece all this together, there is actually more evidence to indicate that it was a different child to the one who's heel stick & saliva samples were retrieved from the UK.

2 It was the same child who's heel stick & saliva samples came from the UK, but there was a need to hide her true DNA.

These 2 considerations boil down to one thing, there is an issue with this child’s genetic make up, and that could be something as simple as blood chimeraism, or something as complex as artificial embryo twinning or a combination of both .

We know or have been told, that Madeleine had a genetic defect, though we haven’t really considered it as such. But her coloboma, if she had one, was a genetic defect.

Were there any other things the Mc's haven’t told us about Madeleine's genetics? Have doctors been playing God ?

''A match between a crime scene sample and an individual would be a very rare event if the individual was not the true source of the crime scene sample.''

I think every attempt was made to confuse Madeleine' DNA, hence the reports of Amelie wearing M’s clothes, and it wouldn't surprise me to learn JT's child wore some of her clothes too

It would be relatively easy for someone to mix up two or three peoples DNA, simply by getting other children to wear the missing child’s clothing for a day or so.

It would be easy to get rid of any hairs from a hairbrush, mix DNA on a toothbrush, change & wash bedding, confuse people about which toys or books belonged to which child etc.

It wouldn't surprise me if the famous Cuddle-Cat didn't have any traces of MM's DNA on it , hence the need to wash it - Not to get rid of any genetic traces of her, but to cover themselves should the police have seized it and discovered that there was nothing on it, or nothing that matched the assembled profile sample - prior to it being washed.

All this is possible - but it still leaves us with the big question - Why? Why the need to go to such lengths to hide this child’s DNA?

What difference would it have made if they found an abundance of her DNA in PDL which provided the authorities with a genetic profile? ..........

I've no doubts about Eddie and Keela's abilities, and I believe a body was present in that apartment and possibly the hire vehicle. Common sense tells us that there is only one person that we know of that is missing. And the investigation has shown that no one else died in the apartment.
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